Serum transferrin (total iron-binding capacity) in evaluation of iron status

Total iron-binding capacity calculated from serum transferrin concentration or serum iron concentration and unsaturated iron-binding capacity.

in borderline cases. The routine use of other biomar-ker assays, such as HFABP in conjunction with tGST activity, could provide complementary information on the potential allograft " viability ". This is illustrated by the fact that there was better segregation of donor categories (controlled vs uncontrolled) with HFABP than with tGST activity. This finding therefore warrants continued evaluati...

Indicators of the iron status of populations: free erythrocyte protoporphyrin and zinc protoporphyrin; serum and plasma iron, total iron binding capacity and transferrin saturation; and serum transferrin receptor

Simplified determination of serum iron and total iron-binding capacity.

2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10,when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint det...

transferrin by iron-binding capacity

SYNOPSIS Two criteria which ought to be satisfied by an acceptable method for the estimation of serum transferrin by iron-binding capacity are enunciated. A screening procedure is described which involves the sequential quantitative use of ion exchange and gel filtration. Its use in testing the extent to which two published methods fulfil the recommended criteria is described. Both methods give...

Serum iron and total iron-binding capacity as measured radiometrically.

With the increasing availability of programmable calculators and minicomputers, it is desirable to demystify radioimmunoassay calculations, and make them available to laboratories lacking massive computing facilities. Recently I modified the Hewlett-Packard 67/97 logit-log radioimmunoassay program to include weighting (submitted to the HP Program Library). I provide here a description of this p...